Región de Murcia
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15th international cyclodextrin symposium

The complexation of daidzein with native -cyclodextrins (-CDs) and modified hydroxypropyl--cyclodextrins (HP--CDs) was studied at pH 7.0. The complexation constant between daidzein and the two types of CDs was calculated by using fluormetric method.

When increasing concentrations of CDs were added to the reaction medium, the fluorescence intensity increased until a maximum was reached (Figure 1 A). This increase was due to the formation of inclusion complexes between daidzein and CDs. The representation of 1/(F-F0) vs 1/[CDs] (double reciprocal plot), known as a Benesi-Hildebrand plot (Figure 1B), leads to a straight line in both cases, indicating the 1:1 stoechiometry of the complexes. The Kc values obtained showed that HP--CDs (Kc of 3072  352 M-1), were more effective in the complexation of daidzein than -CDs (Kc of 1115  102 M-1).

Fluorescence intensities of daidzein at exc 260 nm and emi 465 nm, versus different concentrations of CDs. () -CDs, () HP--CDs. (B) Benesi-Hildebrand plot for daidzein-CDs. () -CDs and () HP--CDs.
FIGURE 1: (A) Fluorescence intensities of daidzein at exc 260 nm and emi 465 nm, versus different concentrations of CDs. () -CDs, () HP--CDs. (B) Benesi-Hildebrand plot for daidzein-CDs. () -CDs and () HP--CDs.

KEYWORDS: Daidzein, complexation constant, cyclodextrins, fluorimetric method.

Los objetivos por los cuales voy al congreso son los siguientes.

Es el congreso más importante relacionado con el tema de mi investigación y considero que es muy importante asistir al mismo pues me podría dar ideas para solucionar ciertos problemas.

Programa

Intercambio de Conocimientos Científicos

Convocatoria

Asistencia a congresos científicos 2010

Área

Ciencias agrarias y agroalimentarias (CAA) / Tecnología de alimentos (780)

Expediente

14569/AC/10

Investigador

Lucas Abellán, Carmen

Grupo de Investigación

REM